Fig. 6From: Enhanced trypsin thermostability in Pichia pastoris through truncating the flexible regionCD spectra of WT and variants (D3, D5, D7, D9, and D11) were measured at room temperature (25 °C). The concentration of protein used for the far-UV CD spectrum (190–260 nm) was 0.2 mg/mL. Each enzyme equilibrated in Tris–HCl buffer (10 mM, pH 8.0)Back to article page