Table 2 The deficient strains
Strains | Relevant properties | Genes | Gene description | Knockout length (bp) |
|---|---|---|---|---|
ATCC6051∆1 | ∆spoIIAC | spoIIAC | The gene of RNA polymerase sigma-F factor | 371 |
ATCC6051∆2 | ∆spoIIAC∆srfAC | srfAC | The gene of surfactin synthase subunit | 1024 |
ATCC6051∆3 | ∆spoIIAC∆srfAC∆aprE | aprE | The gene of serine protease AprE | 189 |
ATCC6051∆4 | ∆spoIIAC∆srfAC∆aprE∆nprE | nprE | The gene of bacillolysin | 623 |
ATCC6051∆5 | ∆spoIIAC∆srfAC∆aprE∆nprE∆nprB | nprB | The gene of neutral protease B | 297 |
ATCC6051∆6 | ∆spoIIAC∆srfAC∆aprE∆nprE∆ nprB∆epr | epr | The gene of minor extracellular protease Epr | 326 |
ATCC6051∆7 | ∆spoIIAC∆srfAC∆aprE∆nprE∆nprB∆epr∆mpr | mpr | The gene of glutamyl endopeptidase Mpr | 404 |
ATCC6051∆8 | ∆spoIIAC∆srfAC∆aprE∆nprE∆nprB∆epr∆mpr∆bpr | bpr | The gene of bacillopeptidase F | 1786 |
ATCC6051∆9 | ∆spoIIAC∆srfAC∆aprE∆nprE∆nprB∆epr∆mpr∆bpr∆vpr | vpr | The gene of extracellular serine protease Vpr | 701 |
ATCC6051∆10 | ∆spoIIAC∆srfAC∆aprE∆nprE∆nprB∆epr∆mpr∆bpr∆vpr∆wprA | wprA | The gene of cell wall-associated protease WprA | 748 |
ATCC6051∆11 | ∆spoIIAC∆srfAC∆aprE∆nprE∆nprB∆epr∆mpr∆bpr∆vpr∆wprA∆hag | hag | The gene of flagellin | 698 |