Fig. 6

Extracellular BTL2 activity with the different secretion signals. This experiment was performed as in Fig. 2a, except that the secreted protein was BTL2. The abbreviations for the constructs are as in Fig. 2. a BTL2 lipolytic activity in the culture medium for the different secretion signals. The activity values were normalized by setting the value for the pre-pro-αf reference strain to 1. b Final OD₆₀₀ values of the cultures at the end of the culture period. Two cultures of the parental X-33 wild-type strain were processed in parallel as a control. c Same as a, except that each lipolytic activity value was divided by the OD₆₀₀ at the end of the incubation. The values were normalized by setting the value for the pre-pro-αf reference strain to 1