Fig. 1
From: An improved secretion signal enhances the secretion of model proteins from Pichia pastoris
Constructs used in this study. Blue is the α-factor signal sequence, green is the α-factor pro region, yellow is the Ost1 signal sequence, and red is E2-Crimson. The wild-type α-factor pro region variant designated here as pro-α-factor contains Leu42. The pro-α-factor(I) or “Invitrogen” variant contains Ser42 as well as the Asp83-to-Glu mutation (see Additional file 1: Figure S1), as represented by the asterisks. The “MUT1″ and “MUT2” variants of the α-factor pro region contain individual Ser42 and Glu83 mutations, respectively, relative to the Leu42 variant