Restoration of biofuel production levels and increased tolerance under ionic liquid stress is enabled by a mutation in the essential Escherichia coli gene cydC

Table 1 Evaluation of [EMIM]OAc sensitivity in E. coli single gene deletion strains

Deletion mutants corresponding to candidate upregulated genes identified from shotgun proteomics were obtained from the Keio collection. Precultures of the indicated genotype were serially diluted into wells of a microtiter plate and spotted onto solid LB agar media with or without 100 mM [EMIM]OAc. Growth of each E. coli deletion strain was compared to growth of a wild-type E. coli of the same strain background (BW25113). No pre-existing growth defects were observed on LB agar plates without supplemental [EMIM]OAc

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