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Table 3 Relative 13C enrichment of all seventeen carbon atoms of riboflavin produced by A. gossypii B2 from different 13C-labeled tracer substrates

From: Metabolic flux analysis in Ashbya gossypii using 13C-labeled yeast extract: industrial riboflavin production under complex nutrient conditions

C-atom Chemical shift (ppm) Corrected relative enrichment (%)
Nat. lab. precursors [13C] For [13C2] Gly [U13C] YE [13C5] Glu
0 h 0 h 48 h 0 h 32 h 0 h
2 155.5 0.0 3.7 11.6 0.0 16.2 1.1
4 159.9 0.0 0.0 0.0 0.0 12.1 1.8
4a 136.8 0.0 0.0 0.0 72.1 19.6 0.0
5a 134 0.0 0.0 0.0 0.0 4.2 1.9
6 130 0.0 0.0 0.0 0.0 4.5 2.3
7 137.1 0.0 0.0 0.0 0.0 3.0 1.8
18.8 0.0 0.0 0.0 0.0 3.0 1.7
8 146 0.0 0.0 0.0 0.0 4.0 1.9
20.8 0.0 0.0 0.0 0.0 4.0 2.6
9 117.4 0.0 0.0 0.0 0.0 3.1 0.7
9a 132.1 0.0 0.0 0.0 0.0 3.0 0.3
10a 150.8 0.0 0.0 0.0 71.6 19.7 0.0
1′ 47.3 0.0 0.0 0.0 0.0 9.9 2.9
2′ 68.8 0.0 0.0 0.0 0.0 8.7 4.1
3′ 73.6 0.0 0.0 0.0 0.0 7.7 3.0
4′ 72.8 0.0 0.0 0.0 0.0 8.1 3.9
5′ 63.4 0.0 0.0 0.0 0.0 7.3 1.6
  1. The labeling was analyzed by 13C NMR. The measurement data (uncorrected, Additional file 1: Table S6) were corrected values for naturally occurring isotopes and dilution through naturally labeled pre-culture medium. The time refers to the respective tracer addition. Data denote mean values of three independent replicates with a mean standard deviation of 8%
  2. For, formate; Glu, glutamate; Gly, Glycine; Ser, serine; YE, yeast extract