Fig. 5
Carbon fluxes during growth of A. gossypii B2 on rapeseed oil and complex medium. Fluxes are given in mmol g −1CDW  h−1 and are normalized to the substrate uptake rate (0.43 ± 0.06 mmol g−1 h−1). Flux calculations were derived from parallel 13C tracer studies with [13C2] glycine, [13C] formate, [13C5] glutamate, and [U13C] yeast extract (Additional file 1: Tables S2, S3). The arrow thickness is proportional to the corresponding flux. The direction of net fluxes is indicated by size of arrow head. Dashed arrows represent fluxes into biomass. Reactions at the OAA/MAL and PEP/PYR node could not be resolved by this approach and represent lumped fluxes. The rate of the pyruvate dehydrogenase could not be determined. In order to simplify the figure, only GTP uptake is depicted as immediate riboflavin precursor. Other nucleotides as well as cysteine and methionine, which are also taken up from the medium, are not depicted. Note that the conversion of citrate to isocitrate via aconitase most likely does not occur in the peroxisome [68]. 3PG, 3-phosphoplycerate; AcCoAP/M, peroxisomal/mitochondrial acetyl-CoA; AKG, α-ketoglutarate; ALA, alanine; ARG, arginine; ASN, asparagine; ASP, aspartate; BM, biomass; CH2-THF, 5,10-methylenetetrahydrofolate; CHO-THF, 10-formyltetrahydrofolate; CIT, citrate; DHAP, dihydroxyacetone phosphate; E4P, erythrose 4-phosphate; F6P, fructose 6-phosphate; FA, fatty acids (here three FA with an average chain length of 17.3 carbon atoms); FOR, formate; G3P, glyceraldehyde 3-phosphate; G6P, glucose 6-phosphate; GAR, glycineamide ribonucleotide; GLN, glutamine; GLU, glutamate; GLY, glycine; GLYC, glycerol; GLYOX, glyoxylate; GTP, guanosine triphosphate; IMP, inosine monophosphate; MAL, malate; OAA, oxaloacetate; PEP, phosphoenolpyruvate; PYR, pyruvate; R5P, ribose 5-phosphate; Ru5P, ribulose 5-phosphate; S7P, sedoheptulose 7-phosphate; SER, serine; THF, tetrahydrofolate; Xu5P, xylulose 5-phosphate; YE, yeast extract