Table 4 Strains, plasmids and primers used in this study

Strain

 G. oxydans 621H

G. oxydans DSM2343, wild type, Cef^r

DSMZ^b

 ∆GOX1068

gox1068 deletion strain of G. oxydans 621H

This study

 ∆GOX0854

gox0854 deletion strain of G. oxydans 621H

This study

 ∆GOX1068∆GOX0854

gox1068 and gox0854 deletion strain of G. oxydans 621H, Cef^r

This study

 ∆GOX2088

gox2088 deletion strain of G. oxydans 621H

This study

 E. coli DH5α

F⁻ supE44 ∆lacU169 (φ80 lacZ∆M15) hsdR17 recA1 endA1 gyrA96 thi⁻¹ relA1 λ⁻

Novagen

 E. coli HB101

F⁻ mcrB mrr hsdS20(rB⁻mB⁻) recA13 supE44 ara14 galK2 lacY1 proA2 rpsL20(Sm^r) xy15 λ^-leu mtl1; used for triparental mating as the helper strain

Invitrogen

Plasmid

 pK18mobsacB

Suicide plasmid, oriColE1 Mob⁺, lacZα, sacB, Km^r

Schäfer et al.

 pK18mobsacB-∆GOX1068

pK18mobsacB with partial gox1068 of G. oxydans 621H for deletion

This study

 pK18mobsacB-∆GOX0854

pK18mobsacB with partial gox0854 of G. oxydans 621H for deletion

This study

 pK18mobsacB-∆GOX1068∆GOX0854

pK18mobsacB with partial gox1068 and gox0854 of G. oxydans 621H for deletion

This study

 pK18mobsacB-∆GOX2088

pK18mobsacB with partial gox2088 of G. oxydans 621H for deletion

This study

Primers

 QGOX1068.f

CCAGAATTCGATGACTTCTGGTCTACTGAC (EcoRI)

This study

 QGOX1068.r

TAAGGATCCTCAGGGGTGATCCGCGGTCG (BamHI)

This study

 GOX1068up.r.

GAGCAGAACGTCTGAGTTGGTCGTGGCGTA

This study

 GOX1068down.f

TACGCCACGACCAACTCAGACGTTCTGCTC

This study

 QGOX0854.f

CCAGAATTCGATGCGCAGATCCCATCTTCT (EcoRI)

This study

 QGOX0854.r

TATAAGCTTTCAGCCCTTGTGATCAGGCA (HindIII)

This study

 GOX0854up.r.

GCCCCACGGACCATTCTGGCACTCTGCTGC

This study

 GOX0854down.f

GCAGCAGAGTGCCAGAATGGTCCGTGGGGC

This study

 GOXQ2088.f

CGCGGATCCATGTCATCGATTCACGAGGTTTCA (BamHI)

This study

 QGOX2088.r

CCCAAGCTTTCAGGCGACGTTCTGACGTGCGA (HindIII)

This study

 GOX2088up.r.

CGAAAACCGACAGGAAGCCGGCTGTCCTGC

This study

 GOX2088down.f

GCAGGACAGCCGGCTTCCTGTCGGTTTTCG

This study