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Fig. 5 | Microbial Cell Factories

Fig. 5

From: Evaluation of GFP reporter utility for analysis of transcriptional slippage during gene expression

Fig. 5

Slippage efficiency of the poly(T6/T7)gfp-1 expressed genes presented as RFU and after normalization to fluorescence of corresponding GFP reference. a Sequence details of compared hybrids constructs with poly(T6) and poly(T7) (underlined) after transcriptional slippage by a single T insertion (italic red). b Fluorescence of hybrids shown in arbitrary units (white bars) and relative fluorescence values after normalization to fluorescence of a proper in-frame positive control values (black bars), respectively. Errors bars represent standard deviations from three independent experiments

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