Fig. 7

a Sugar-accumulation and cell death measured by FCM for three cultivations at different q_s,Glu. Lowest q_s,Glu shows no cell lysis and accumulation; b specific titer of the recombinant protein fused to N-pro. Very high expression can be seen for the high q_s,Glu until 6 h with decreasing q_s,Glu also decreases product titer; c size of the IB beads. q_s,Glu = 0.41 g/g/h and q_s,Glu = 0.39 g/g/h are very similar. A very steep increase is followed by a steady state; q_s,Glu = 0.30 g/g/h shows increase over time, while size for q_s,Glu = 0.10 g/g/h is only detectable at 10 and 12 h of induction; d purity depicts clear dependence of all different q_s,Glu setpoints, increasing the IB purity with higher q_s,Glu