Production of p-amino-l-phenylalanine (l-PAPA) from glycerol by metabolic grafting of Escherichia coli

Table 3 Comparison of l-PAPA yields in next generation strains

E. coli strains	Final OD (600 nm)	Yield l-PAPA/glycerol (g g⁻¹)	Final l-PAPA titer (mg l⁻¹)
FUS 4/pC53BC/pJNT-aroFBL	3.77 ± 0.25	0.04	202.7 ± 4.5
FUS 4.7/pC53BC/pJNT-aroFBL	3.66 ± 0.13	0.09	449.4 ± 29.4
FUS 4.7R/pC53BC/pJNT-aroFBL	3.86 ± 0.14	0.11	534.1 ± 24.1

Double auxotroph strains carrying two plasmids (pC53BC and pJNT-aroFBL) were grown in shake flasks in minimal media with 5 g l⁻¹ glycerol and supplemented by l-Phe and l-Tyr (0.04 g l⁻¹ each) and appropriate antibiotics. Induction with IPTG (0.5 mM final concentration) was at OD₆₀₀ of ca. 0.6 and strains were further incubated until a total cultivation time of 48 h. The cultivations were performed in triplicate and mean values and standard deviations are given

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