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Table 1 List of E. coli strains, plasmids and oligonucleotides

From: Production of p-amino-l-phenylalanine (l-PAPA) from glycerol by metabolic grafting of Escherichia coli

Strains Characteristics Reference/origin
E. coli DH5α φ80lacZΔM15 Δ(lacZYA-argF)U169 recA1 endA1 hsdR17(rK−, mK +) phoA supE44 λthi-1 gyrA96 relA1 Invitrogen
E. coli LJ110 Wildtype W3110 (F−, λ−, IN (rrnD-rrnE) 1, rph-1) [53]
E. coli FUS 4 LJ110 Δ(pheA-tyrA-aroF) Δlac::Ptac::aroFBL+ [15]
E. coli FUS4.7 FUS4 Δrbs::Ptac::glpX+ Δgal::Ptac::tktA+ [15]
E. coli FUS4.7R FUS4.7ΔtyrR::FRT This study
Plasmids
 pJNT522 Ptac, RBS, KanR This study, see Additional file 1
 pJNT-aroFBL Ptac::aroFE.c., aroBE.c, aroLE.c., KanR This study
 pJF119EH Ptac, RBS, AmpR, lacI [54]
 pC53 pJF119EH, Ptac::pabABC.g., AmpR, lacI [41]
 pC53BC pJF119EH, Ptac::pabABC.g.-papBCS.v., AmpR, lacI This study
 pMK-papBC Cloning vector including the codon optimized genes of papBCS.v, KanR Thermo Fisher (GeneArt) custom synthesis
 pKD46 λ Red disruption system (γ, β, exo under control of ParaBAD), AmpR [55]
 pCP20 FLP+, λcI857+, λ ρR Repts, AmpR, CmR [56]
 pCAS30-FRT-cat-FRT pJF119ΔN, P. ananatis crtE gene, FRT-sites, AmpR, CmR, source of cat-gene cassette [57]
 pJeM2 rhaR rhaS rhaPBAD, eGFP, mob KanR [58]
Primers Sequence  
Del-tyrR-Fw 5′-ATCATCATATTAATTGTTCTTTTTTCAGGTGAAGGTTCCCTGTGTAGGCTGGAGCTGCTTCG-3′
Del-tyrR-Rw 5′-AATATGCCTGATGGTGTTGCACCATCAGGCATATTCGCGCCATATGAATATCCTCCTTAG-3′
Ko-tyrR-Fw 5′-TGACGGCACGACTCGGGATTAAAG-3′
Ko-tyrR-Rw 5′-AACAACGTTATCACCCTC TCCACT-3′