Table 1 List of E. coli strains, plasmids and oligonucleotides

E. coli DH5α

φ80lacZΔM15 Δ(lacZYA-argF)U169 recA1 endA1 hsdR17(rK−, mK +) phoA supE44 λ− thi-1 gyrA96 relA1

Invitrogen

E. coli LJ110

Wildtype W3110 (F−, λ−, IN (rrnD-rrnE) 1, rph-1)

[53]

E. coli FUS 4

LJ110 Δ(pheA-tyrA-aroF) Δlac::P_tac::aroFBL+

[15]

E. coli FUS4.7

FUS4 Δrbs::P_tac::glpX+ Δgal::P_tac::tktA+

[15]

E. coli FUS4.7R

FUS4.7ΔtyrR::FRT

This study

Plasmids

 pJNT522

P_tac, RBS, Kan^R

This study, see Additional file 1

 pJNT-aroFBL

P_tac::aroF_E.c., aroB_E.c, aroL_E.c., Kan^R

This study

 pJF119EH

P_tac, RBS, Amp^R, lacI

[54]

 pC53

pJF119EH, P_tac::pabAB_C.g., Amp^R, lacI

[41]

 pC53BC

pJF119EH, P_tac::pabAB_C.g.-papBC_S.v., Amp^R, lacI

This study

 pMK-papBC

Cloning vector including the codon optimized genes of papBC_S.v, Kan^R

Thermo Fisher (GeneArt) custom synthesis

 pKD46

λ Red disruption system (γ, β, exo under control of P_araBAD), Amp^R

[55]

 pCP20

FLP+, λ_cI857+, λ ρR Rep^ts, Amp^R, Cm^R

[56]

 pCAS30-FRT-cat-FRT

pJF119ΔN, P. ananatis crtE gene, FRT-sites, Amp^R, Cm^R, source of cat-gene cassette

[57]

 pJeM2

rhaR rhaS rhaPBAD, eGFP, mob Kan^R

[58]

Del-tyrR-Fw

5′-ATCATCATATTAATTGTTCTTTTTTCAGGTGAAGGTTCCCTGTGTAGGCTGGAGCTGCTTCG-3′

Del-tyrR-Rw

5′-AATATGCCTGATGGTGTTGCACCATCAGGCATATTCGCGCCATATGAATATCCTCCTTAG-3′

Ko-tyrR-Fw

5′-TGACGGCACGACTCGGGATTAAAG-3′

Ko-tyrR-Rw

5′-AACAACGTTATCACCCTC TCCACT-3′