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Fig. 4 | Microbial Cell Factories

Fig. 4

From: Production of p-amino-l-phenylalanine (l-PAPA) from glycerol by metabolic grafting of Escherichia coli

Fig. 4

Fed-batch production of l-PAPA in a 30 l bioreactor. Cell dry weight (CDW, empty circle), glycerol consumption (g l−1, empty triangle) and l-PAPA concentration (g l−1, empty square) were monitored for E. coli FUS4.7R/pC53BC/pJNT-aroFBL. The strain was grown in minimal media with an initial concentration of 8.1 g l−1 glycerol. The glycerol feed started after 11.4 h (red solid line, first pulse of l-Phe and l-Tyr and induction with 0.5 mM IPTG) and the feed was adjusted to maintain a concentration of 0.6–1.0 g l−1 glycerol. A second pulse of l-Phe and l-Tyr (dotted line) and a third pulse of l-Phe and l-Tyr (dash line) were fed to allow biomass formation. The result of one fermentation is presented with mean values and deviations of three technical replicates

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