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Fig. 3 | Microbial Cell Factories

Fig. 3

From: Production of p-amino-l-phenylalanine (l-PAPA) from glycerol by metabolic grafting of Escherichia coli

Fig. 3

Glycerol fed-batch cultivation of E. coli FUS4.7 R/pC53BC/pJNT-aroFBL in shake flasks. The initial glycerol concentration was 5 g l−1. After 24 h of cultivation the cultures were induced with 0.5 mM IPTG (final concentration) and the shake flasks were transferred from 37 to 30 °C before adding pulses of ~ 5 g l−1 of glycerol every 12 h. To adjust the pH, sodium bicarbonate (30 mM) was added every 24 h as well as 15 mM of ammonium sulfate. The concentrations of glycerol (empty circles) and, l-PAPA (empty squares) were determined by HPLC. OD600 values are presented as filled squares. The cultivations were performed in triplicate and the mean values and standard deviations are given

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