Skip to main content

Table 1 Oligonucleotide primers used in this study

From: Evaluation of cell-surface displayed synthetic consensus dengue EDIII cells as a potent oral vaccine candidate

Primers

Sequence (5′–3′)a

Co1-scEDIII-AGA construction

 Ramyl-F

GGATCCGCATGCAGGTGC

 Ramyl-Co1 OR

TAGCTGGTAGTTGATGGAAAGACCCGGCTGTCAAGT

 Co1-scEDIII F

TCAACTACCAGCTAGAAGTCCACTACCAAAAGGAATGTCT

 scEDIII-AGA OF

TAAAAAGGGTTCCTCAGCCAAAAGCTCT

 scEDIII-AGA OR

AGAGCTTTTGGCTGAGGAACCCTTTTTA

 AGA-R

GTCGACGCTTAGAATAGCAGGTACGACAA

scEDIII-Co1 construction

 

 scEDIII-Co1-F

CGGGATCCCGATGAAAGGAATGTCTTACGCA

 scEDIII-Co1-R

GTCGACCTATGGTAGTGGACTTCTAGCTGGTAG TTGATGGAAAGATGAGGAACCCT

  1. aUnderlined nucleotides indicate engineered restriction sites used in cloning
Back to article page

Microbial Cell Factories

ISSN: 1475-2859

Contact us