Fig. 6

Mucosal sIgA responses upon oral immunization. scEDIII-specific fecal sIgA was induced in mice immunized orally with recombinant scEDIII-Co1 and Co1-scEDIII-AGA at 4 days after the last immunization until day 32, observed at weekly intervals. Dengue-specific fecal sIgA levels were also observed on day 46, 3 days after booster immunization with purified E. coli expressed scEDIII protein. An unpaired Student’s t-test was used to calculate p-values, and *p < 0.05 indicates significant differences between the groups