Fig. 1
From: Genome alterations associated with improved transformation efficiency in Lactobacillus reuteri
Transformation of pSH71 derivatives increases transformation efficiency of LR 6475. Transformation efficiency of LR 6475 and mutants generated by plasmids transformation. LJO1, LJO3 and LJO8 were cured from their original plasmids and retransformed with pJP067 (a), pSIP411 (b) and pNZ8048 (c). CMR: chloramphenicol resistant, EMR: erythromycin resistant. Data represents the averages of three independent experiments and error bars represent standard deviation. (*, **, *** indicates the significant difference at p < 0.05 p < 0.01 and p < 0.001, respectively). d Measurement of the colony size after transformation of pNZ8048, including LR 6475 (e) and LJO3 (f). Colony forming Units (CFU) were divided based on their radius as small (0–6 units) and big (7–18 units). Radius was a measure of the pixel size. Data represents the averages of three independent experiments