Table 1 The plasmids and bacterial strains used in this study

Strains

 B. subtilis

  168

trpC2

[47]

  168_sfGFP(Sp)

trpC2, amyE::P_hyspank-sfgfp(Sp) spc^r

[32]

  168_sfGFP(Sp)_CodY^R214C

trpC2, codY^R214C cm^r, amyE::P_hyspank-sfgfp(Sp) spc^r

This study

  168_sfGFP(Sp)_CcpA^T19S

trpC2, ccpA^T19S km^r, amyE::P_hyspank-sfgfp(Sp) spc^r

This study

  168_sfGFP(Sp)_CodY^R214CCcpA^T19S

trpC2, codY^R214C cm^r, ccpA^T19S km^r, amyE::P_hyspank- sfgfp(Sp) spc^r

This study

 E. coli

  MC1061

F⁻, araD139, Δ(ara-leu)7696, Δ(lac)X74, galU, galK, hsdR2, mcrA, mcrB1, rspL

[48]

Plasmids

 pCH3_CcpA^T19S

pUC18_aroA_ccpA^T19S_km^r_ytxD

[34]

 pJV153

pUC18_clpY_codY^R214C_cm^r_flgB

[34]