Fig. 2

The expression of sfGFP(Sp) in various B. subtilis strains. B. subtilis WT, CodY^R214C, CcpA^T19S, CodY^R214CCcpA^T19S harboring amyE::P_hyspank-sfgfp(Sp) were grown in flasks with LB supplemented with 1.0% glucose and 0.1 mM IPTG under the same growth conditions (37 °C, 220 rpm). Samples were harvested for both fluorescence and OD₆₀₀ measurement per hour. a Flow cytometric analysis of GFP expression. Dotted lines were placed at 10³ Arbitrary Units (AU) to serve as a reference of the fluorescence distributions. b The mean fluorescence intensity of the whole population over time. c The optical density at 600 nm of various strains was measured by spectrophotometry. For WT, CcpA^T19S, the exponential phase is 1–4 h, and start the stationary phase from the point of 4 h; for CodY^R214C and CodY^R214CCcpA^T19S, the exponential phase is 1–5 h, and enter the stationary phase from the time point of 5 h