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Table 3 Kinetic parameters of erythrose reductase encoded by gene g141.t1 (ER10), g3023.t1 (ER25) and g801.t1 (ER27)

From: Identification, characterization of two NADPH-dependent erythrose reductases in the yeast Yarrowia lipolytica and improvement of erythritol productivity using metabolic engineering

Enzymes Substrate/cofactor Km (mM) Vmax (μmol/min) Kcat (s−1) Kcat/Km (mM−1s−1)
ER10 d-Erythrose/NADPH 11.46 8.17 11.43 0.98
ER10 Erythritol/NADP+ 159.7 0.62 0.86 0.005
ER25 d-Erythrose/NADPH 18.06 14.58 19.70 1.09
ER27 d-Erythrose/NADPH 25.99 12.01 15.8 0.61
  1. Cofactors (NADPH or NADP+) concentrations were 2 mM. Km is for d-erythrose or erythritol