Identification, characterization of two NADPH-dependent erythrose reductases in the yeast Yarrowia lipolytica and improvement of erythritol productivity using metabolic engineering

Table 3 Kinetic parameters of erythrose reductase encoded by gene g141.t1 (ER10), g3023.t1 (ER25) and g801.t1 (ER27)

Enzymes	Substrate/cofactor	K_m (mM)	V_max (μmol/min)	K_cat (s⁻¹)	K_cat/K_m (mM⁻¹s⁻¹)
ER10	d-Erythrose/NADPH	11.46	8.17	11.43	0.98
ER10	Erythritol/NADP⁺	159.7	0.62	0.86	0.005
ER25	d-Erythrose/NADPH	18.06	14.58	19.70	1.09
ER27	d-Erythrose/NADPH	25.99	12.01	15.8	0.61

Cofactors (NADPH or NADP⁺) concentrations were 2 mM. K_m is for d-erythrose or erythritol

ISSN: 1475-2859