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Table 5 Phosphorylation state of EIIAGlc during two-stage cultivations

From: Type and capacity of glucose transport influences succinate yield in two-stage cultivations

Strain

EIIAGlc-P [%]

EIIAGlc-P [%]

Aerobic stage

Anaerobic stage

MG1655

4.2 ± 1.3

4.9 ± 2.1

KBM151101

2.1 ± 0.8

4.5 ± 2.3

SB2

91.3 ± 1.3

80.3 ± 3.1

KBM1673

72.1 ± 12.5

23.3 ± 6.0

SB2/pPckA

83.4 ± 6.0

71.5 ± 3.5

KBM1673/pPckA

61.4 ± 12.3

56.9 ± 15.3

  1. Shown are the EIIAGlc phosphorylation levels in the different strains during the aerobic and anaerobic phase of two-stage cultivations. Samples were taken during the exponential phase of aerobic growth and after 4 h in the anaerobic phase. Data are derived from at least two independent experiments. Data from single growth curves as well as exemplary pictures of Western Blots are shown in Additional file 1: Table S2 and Figure S1
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Microbial Cell Factories

ISSN: 1475-2859

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