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Table 1 Strains and plasmids used in this study

From: Type and capacity of glucose transport influences succinate yield in two-stage cultivations

Strain

Relevant genotype

Source or reference

MG1655

Wildtype

FRB426 (Blattner lab)

KBM151101

MG1655 ΔackA-pta ΔadhE ΔldhA ΔlacI-A

This study

SB2

MG1655 ΔackA-pta ΔadhE ΔldhA ΔlacI-A ΔptsG

This study

KBM1673

MG1655 ΔackA-pta ΔadhE ΔldhA ΔlacI-A ΔptsG

attphi80::pAH162_scrKp_glfZ.mobilis

This study

Plasmid

 pPckA

pckA from E. coli MG1655 in pRR48

This study

 pEcPpc

ppc from E. coli in pTrc99A

[32]