Fig. 4
From: Type and capacity of glucose transport influences succinate yield in two-stage cultivations
Analysis of pckA and ppc expression by Real Time RT PCR. Shown are the relative expression levels of pckA (blue) and ppc (red) determined for the different strains under aerobic and anaerobic conditions. Samples were taken from the exponential growth phase of the aerobic stage and after 4 h (mutant strains) or 2 h (MG1655 in the anaerobic stage. Gene expression was analysed by the ∆∆Ct method with normalization to three housekeeping genes and to MG1655 under the respective condition. Y-axes are plotted logarithmically to be able to show also downregulation