Fig. 5

In vitro characterization of TubG as a Nudix hydrolase responsible for the final hydrolysis step. a Schematic of the TubG-catalyzed reaction using the analog of 5, AMP, as substrate. b HPLC traces of the TubG reaction. Std, the authentic standards of AMP and adenosine; TubG, HPLC traces of the TubG reaction with AMP as substrate; N.C, EIC analysis of the reaction with AMP as substrate added but without TubG added. c Relative activity of TubG with AMP as substrate and different divalent cations as metallic cofactor