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Fig. 4 | Microbial Cell Factories

Fig. 4

From: Discovery and characterization of the tubercidin biosynthetic pathway from Streptomyces tubercidicus NBRC 13090

Fig. 4

In vitro characterization of TubD as an NADPH-dependent reductase. a Schematic of the TubD-catalyzed reaction in the presence of the redox cofactor NADPH. b LC–MC analysis of the TubD reaction. Std, EIC analysis of the authentic standards of IMP and GMP; +NADPH, EIC analysis of the TubD reaction with NADPH as redox cofactor; +NADH, EIC analysis of the TubD reaction with NADH as redox cofactor; N.C, EIC analysis of the reaction with NADPH and substrate added but lacking the enzyme TubD

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