Table 1 Strains and plasmids used in this study

Strains

 E. coli XL1-Blue

recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac [FA1proAB lacI^qZΔM15 Tn10 (Tet^R)]

Stratagene

 C. glutamicum strains KCTC 1446, 1447, 1852, 3017

Glutamate producers

KCTC

 C. glutamicum ATCC 13032

Wild type

ATCC

 C. glutamicum H30GD13032

C. glutamicum ATCC 13032 harboring pCES208H30 GadBmutXylAB

This study

 C. glutamicum H36GD13032

C. glutamicum ATCC 13032 harboring pCES208H36 GadBmutXylAB

This study

 C. glutamicum H30GD1447

C. glutamicum KCTC 1447 harboring pCES208H30 GadBmutXylAB

This study

 C. glutamicum H36GD1447

C. glutamicum KCTC 1447 harboring pCES208H36 GadBmutXylAB

This study

 C. glutamicum H30GD1852

C. glutamicum KCTC 1852 harboring pCES208H30 GadBmutXylAB

This study

 C. glutamicum H36GD1852

C. glutamicum KCTC 1852 harboring pCES208H36 GadBmutXylAB

This study

 C. glutamicum H36GM1852

C. glutamicum KCTC 1852 harboring pCES208H36 GadBmut

This study

Plasmids

 pCES208H30GFP

pCES208 derivative; P_H30, eGFP, Km^r

[42]

 pCES208H30GFP

pCES208 derivative; P_H36, eGFP, Km^r

[42]

 pHGmut

pCES208 derivative; P_H36, gadB mutant gene from E. coli (Glu89Gln/Δ452-466), Km^r

[27]

 pCES208H30 GadBmutXylAB

pCES208H30GFP derivative; P_H30, gadB mutant gene from E. coli (Glu89Gln/Δ452-466), xylose utilization genes xylAB from E. coli, Km^r

This study

 pCES208H36 GadBmutXylAB

pHGmut derivative; P_H36, gadB mutant gene from E. coli (Glu89Gln/Δ452-466) xylose utilization genes xylAB from E. coli, Km^r

This study