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Fig. 5 | Microbial Cell Factories

Fig. 5

From: Getting value from the waste: recombinant production of a sweet protein by Lactococcus lactis grown on cheese whey

Fig. 5

Production of recombinant MMP9 in the G-M17 and ac-CW based medium. The figure shows a comparison of the western blots of 10 μg of total protein extract of cells of L. lactis NZ9000 clpP− htrA− transformed with the pNZ8148-MMP9 vector after culture in rich medium (a) or in ac-CW supplemented with 0.05% Yeast extract (b). A: no induction; B: 2 h post-induction; C: 4 h post induction; D: 16 h post induction. While protein expression levels seem comparable in the two cases, maximum production appears slightly delayed in the case of the CW derived medium

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Microbial Cell Factories

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