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Fig. 3 | Microbial Cell Factories

Fig. 3

From: Getting value from the waste: recombinant production of a sweet protein by Lactococcus lactis grown on cheese whey

Fig. 3

Production of recombinant MNEI in the ac-CW based medium. Coomassie-stained SDS-PAGE (a) and western blot (b) of the total protein extract (10 μg) from L. lactis NZ9000 carrying the pNZ8148-MNEI-ll and growing on ac-CW + 0.05% yeast extract. A: no induction; B: 2 h post-induction; C: 4 h post induction; D: 16 h post induction; E: MNEI standard, 200 ng; F: MNEI standard, 500 ng

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