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Fig. 1 | Microbial Cell Factories

Fig. 1

From: Getting value from the waste: recombinant production of a sweet protein by Lactococcus lactis grown on cheese whey

Fig. 1

Production of recombinant MNEI in G-M17 medium. Coomassie-stained SDS-PAGE (a) and western blot (b) of the total protein extract (10 μg) from L. lactis NZ9000 carrying the pNZ8148-MNEI-ll vector (A–D) or the empty pNZ8148 vector (E–F). A: no induction; B: 2 h post-induction; C: 4 h post induction; D: 16 h post induction; E: no induction; F: 4 h post induction; G: MNEI standard, 200 ng; H: MNEI standard, 500 ng

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