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Table 6 Secreted production of recombinant β-mannanases in different strains by shake flask

From: Characterization and high-efficiency secreted expression in Bacillus subtilis of a thermo-alkaline β-mannanase from an alkaliphilic Bacillus clausii strain S10

Gene source

Expression hosts

Expression vector

Culture time (h)

Activity (U ml−1)

Productivity (U ml−1 h−1)

Enzyme pH optima

References

B. clausii S10

B. subtilis WB600

pMA5

72

6041

83.9

9.5

This work

B. licheniformis DSM13

B. subtilis 1A751

pMA5

72

2207

30.7

5.5

[5]

B. subtilis WL-3

B. subtilis 168

pJ2788U

15

450

30.0

6.0

[43]

B. pumilus Nsic-2

B. subtilis 1A751

pBNS2

24

7

0.3

7.0

[44]

B. subtilis BCC41051

B. megaterium

pXb

24

359

15.0

7.0

[76]

Bispora sp. MEY-1

P. pastoris GS115

pPIC9

48

64

1.3

1.0–1.5

[59]

A. fumigatus

P. pastoris GS115

pPICZαC

10

61

6.1

5.2

[26]

A. niger BK01

P. pastoris X33

pPICZαA

96

669

7.0

4.5

[27]

B. subtilis MAFIC-S11

P. pastoris X33

pPICZαA

72

1106

15.4

6.0

[42]

A. sulphureus

P. pastoris X33

pPICZαA

96

96

1.0

2.4

[28]

B. subtilis G1

P. pastoris GS115

pPICZαA

108

224

2.1

6.5

[80]

A. niger CBS 513.88

P. pastoris GS115

pHBM905M

192

274

1.4

4.5

[61]

B. subtilis BS5

P. pastoris GS115

pPIC9

96

892

9.3

6.0

[41]

Bacillus sp. N16-5

P. pastoris GS115

pPIC9K

144

1114

7.7

9.0

[45]

Penicillium sp. C6

P. pastoris GS115

pPIC9

120

566

4.7

4.5

[66]

Bacillus sp. N16-5

P. pastoris GS115

pPICZαA

120

32

0.3

10.0

[40]

Penicillium oxalicum

P. pastoris GS115

pPICZαA

144

84

0.6

4.0

[30]

B. circulans NT 6.7

E. coli BL21 (DE3)

pET21d

16

37

2.3

6.0

[38]

B. licheniformis DSM13

E. coli Top10

pFLAG-CTS

4a

13

3.4

6.0–7.0

[37]

Bacillus sp. N16-5

E. coli BL21 (DE3)

pET22b

28

2736

97.7

9.0

[39]

A. aculeatus MRC11624

A. niger D15

pGT

192

16,596b

35.4

3.8

[77]

A. fumigatus

A. sojae

pAN52-4

144

352

2.4

4.5

[26]

A. niger CBS 513.88

Y. lipolytica

pINA1296I

48

255

5.3

4.5

[78]

B. licheniformis DSM13

L. plantarum

pSIP609

12

42

3.5

6.0

[79]

T. harzianum MGQ2

T. reesei OM9414

pUC19

168

2460

14.6

6.0

[31]

  1. aThe time is the induction time
  2. bThe activity defined as the amount of enzyme required to convert one mole of substrate per second and reported in katal (nkat ml−1)
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