Table 4 Substrate specificity and kinetic parameters of BcManA
Substrate | Specific activity (U mg−1) | Relative activity (%) | Mean Km (g l−1) ± SD | Mean kcat (s−1) ± SD | Mean kcat/Km (l s−1 g−1) ± SD |
|---|---|---|---|---|---|
Konjac glucomannan | 2366.2 | 100.0 | 0.62 ± 0.05 | 1238.9 ± 13.2 | 1998.2 ± 21.4 |
Locust bean gum | 2087.1 | 88.2 | 1.68 ± 0.04 | 1376.1 ± 15.1 | 819.1 ± 25.7 |
Guar gum | 428.3 | 18.1 | 3.16 ± 0.06 | 109.9 ± 2.5 | 34.8 ± 1.3 |
Sesbania gum | 615.2 | 26.0 | 5.56 ± 0.11 | 164.7 ± 2.9 | 29.2 ± 1.1 |