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Table 1 Plasmids and strains used in this study

From: Characterization and high-efficiency secreted expression in Bacillus subtilis of a thermo-alkaline β-mannanase from an alkaliphilic Bacillus clausii strain S10

Plasmids or strains

Features

Sources

pUC118

Treated by BamHI and calf intestinal alkaline phosphatase, AmpR

TaKaRa Co., Ltd, China

pET28a

Protein expression vector in E. coli, KanR

Merck Co., Germany

pET28a-Man

pET28a derivative with BcManA gene without signal peptide

This work

pUC57-Simple-PlapS

pUC57 derivative with promoter PlapS sequence

Laboratory preservation

pHT43

E. coli-Bacillus shuttle vector for protein expression in B. subtilis, CmR

BGSC, USA

pMA5

E. coli-Bacillus shuttle vector for protein expression in B. subtilis, KanR

BGSC, USA

pMA5-Man

pMA5 derivative with BcManA gene without signal peptide

This work

pMA5-Man1

pMA5-Man derivative, original signal peptide, SPori

This work

pMA5-Man2

pMA5-Man derivative, SPlipA

This work

pMA5-Man3

pMA5-Man derivative, SPamyE

This work

pMA5-Man4

pMA5-Man derivative, SPlipB

This work

pMA5-Man5

pMA5-Man derivative, SPaprE

This work

pMA5-Man6

pMA5-Man derivative, SPamyL

This work

pMA5-Man2-1

pMA5-Man2 derivative, P43

This work

pMA5-Man2-2

pMA5-Man2 derivative, PlapS

This work

B. clausii S10

DNA source for β-mannanase gene cloning

Laboratory preservation

E. coli DH5α

Host for gene cloning

Transgen Biotech Co., Ltd, China

E. coli BL21(DE3)

Host for protein expression

Transgen Biotech Co., Ltd, China

B. subtilis WB600

Host for protein secreted expression, CmR

Laboratory preservation

B. subtilis WB600-1

WB600 derivative, pMA5-Man, KanR, CmR

This work

B. subtilis WB600-2

WB600 derivative, pMA5-Man1, KanR, CmR

This work

B. subtilis WB600-3

WB600 derivative, pMA5-Man2, KanR, CmR

This work

B. subtilis WB600-4

WB600 derivative, pMA5-Man3, KanR, CmR

This work

B. subtilis WB600-5

WB600 derivative, pMA5-Man4, KanR, CmR

This work

B. subtilis WB600-6

WB600 derivative, pMA5-Man5, KanR, CmR

This work

B. subtilis WB600-7

WB600 derivative, pMA5-Man6, KanR, CmR

This work

B. subtilis WB600-8

WB600 derivative, pMA5-Man2-1, KanR, CmR

This work

B. subtilis WB600-9

WB600 derivative, pMA5-Man2-2, KanR, CmR

This work