Plasmids or strains | Features | Sources |
---|---|---|
pUC118 | Treated by BamHI and calf intestinal alkaline phosphatase, AmpR | TaKaRa Co., Ltd, China |
pET28a | Protein expression vector in E. coli, KanR | Merck Co., Germany |
pET28a-Man | pET28a derivative with BcManA gene without signal peptide | This work |
pUC57-Simple-PlapS | pUC57 derivative with promoter PlapS sequence | Laboratory preservation |
pHT43 | E. coli-Bacillus shuttle vector for protein expression in B. subtilis, CmR | BGSC, USA |
pMA5 | E. coli-Bacillus shuttle vector for protein expression in B. subtilis, KanR | BGSC, USA |
pMA5-Man | pMA5 derivative with BcManA gene without signal peptide | This work |
pMA5-Man1 | pMA5-Man derivative, original signal peptide, SPori | This work |
pMA5-Man2 | pMA5-Man derivative, SPlipA | This work |
pMA5-Man3 | pMA5-Man derivative, SPamyE | This work |
pMA5-Man4 | pMA5-Man derivative, SPlipB | This work |
pMA5-Man5 | pMA5-Man derivative, SPaprE | This work |
pMA5-Man6 | pMA5-Man derivative, SPamyL | This work |
pMA5-Man2-1 | pMA5-Man2 derivative, P43 | This work |
pMA5-Man2-2 | pMA5-Man2 derivative, PlapS | This work |
B. clausii S10 | DNA source for β-mannanase gene cloning | Laboratory preservation |
E. coli DH5α | Host for gene cloning | Transgen Biotech Co., Ltd, China |
E. coli BL21(DE3) | Host for protein expression | Transgen Biotech Co., Ltd, China |
B. subtilis WB600 | Host for protein secreted expression, CmR | Laboratory preservation |
B. subtilis WB600-1 | WB600 derivative, pMA5-Man, KanR, CmR | This work |
B. subtilis WB600-2 | WB600 derivative, pMA5-Man1, KanR, CmR | This work |
B. subtilis WB600-3 | WB600 derivative, pMA5-Man2, KanR, CmR | This work |
B. subtilis WB600-4 | WB600 derivative, pMA5-Man3, KanR, CmR | This work |
B. subtilis WB600-5 | WB600 derivative, pMA5-Man4, KanR, CmR | This work |
B. subtilis WB600-6 | WB600 derivative, pMA5-Man5, KanR, CmR | This work |
B. subtilis WB600-7 | WB600 derivative, pMA5-Man6, KanR, CmR | This work |
B. subtilis WB600-8 | WB600 derivative, pMA5-Man2-1, KanR, CmR | This work |
B. subtilis WB600-9 | WB600 derivative, pMA5-Man2-2, KanR, CmR | This work |