Fig. 2From: Characterization and high-efficiency secreted expression in Bacillus subtilis of a thermo-alkaline β-mannanase from an alkaliphilic Bacillus clausii strain S10SDS-PAGE analysis of purified recombinant BcManA. Lane 1, crude extract from E. coli BL21(DE3) harbouring pET28a-manA; lane 2, supernatant of the crude extract from E. coli BL21(DE3) harbouring pET28a-manA; lane 3, supernatant after heating treatment from E. coli BL21(DE3) with pET28a-manA; lane 4, purified enzyme by His-Tag affinity chromatography; lane M, molecular weight markerBack to article page