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Fig. 1 | Microbial Cell Factories

Fig. 1

From: Fate of the UPR marker protein Kar2/Bip and autophagic processes in fed-batch cultures of secretory insulin precursor producing Pichia pastoris

Fig. 1

Intracellular Kar2/Bip level during glycerol batch growth of P. pastoris X-33 carrying the aox1 promoter-controlled IP gene. Cells were grown in the 2 L bioreactor in a batch procedure with 95 g/L glycerol as carbon source. a The time-dependent relative intracellular Kar2/Bip abundance (gray bars), biomass (optical density: squares), glycerol concentration (triangles), and osmolarity (circles) of the culture broth are given. The dashed vertical lines point to the borders of indicated growth phases. b SDS-PAGE gels and c corresponding immunoblots probing for Kar2/Bip in total cell lysates. M denotes the molecular weight marker and the numbers on bottom of the lanes the post-inoculation sampling time points. Boxed lanes correspond to boxed bars in (a)

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Microbial Cell Factories

ISSN: 1475-2859

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