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Fig. 7 | Microbial Cell Factories

Fig. 7

From: A novel method for transforming the thermophilic bacterium Geobacillus kaustophilus

Fig. 7

pGK1 mobilization with the help of pLS20cat or pLS20catΔoriT. Liquid cultures of the recipient strain MK244 and one of the donor strains (YNB051 or YNB101) were mixed for pGK1 mobilization and plated on LB medium containing kanamycin (K) for transconjugants and no antibiotic for the recipient grown at 65 °C. CFU was determined to calculate mobilization efficiencies. Values are expressed as means with standard deviations from three independent experiments

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