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Fig. 6 | Microbial Cell Factories

Fig. 6

From: A novel method for transforming the thermophilic bacterium Geobacillus kaustophilus

Fig. 6

pGK1 mobilization depending on the growth phase of the donor and recipient. Liquid cultures of the recipient MK244 and the donor YNB051 in different growth phases were mixed for pGK1 mobilization and plated on LB medium containing kanamycin (K) for transconjugants and no antibiotic for the recipient grown at 65 °C. CFU was determined to calculate mobilization efficiencies. Values are expressed as means with standard deviations from three independent experiments. ND not detected (< 1.0 × 10−2 ppm)

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