Table 6 Media used to test PF production by P. fluorescens BD5

KB

King’s B

10 g/L glycerol, 20 g/L proteose peptone, 1.5 g/L K₂HPO₄, 1.5 g/L MgSO₄, 100 mM MOPS

Initial media screening, high-throughput optimization

[59]

KB-M1

Modified King’s B M1

10 g/L glycerol, 2 g/L proteose peptone, 1.5 g/L K₂HPO₄, 1.5 g/L MgSO₄, 100 mM MOPS, various modifications were added (see “Culture media” section)

High-throughput optimization

This work

KB-M2

Modified King’s B M2

40 g/L glycerol, 5 g/L complex additive, 5 g/L Leu, 1.5 g/L K₂HPO₄, 1.5 g/L MgSO₄, 100 mM MOPS, various complex additives were added (see “Culture media” section)

High-throughput optimization

This work

KB-Opt-CCD

Optimized King’s B (CCD model)

67 g/L glycerol, 12.1 g/L tryptone, 9.2 g/L Leu, 0.5 g/L K₂HPO₄, 0.1 g/L MgSO₄, 50 mg/L Fe₂(SO₄)₃, 100 mM MOPS

High-throughput optimization, further culture optimization

This work

KB-Opt

Optimized King’s B

20–100 g/L glycerol, 15 g/L tryptone, 10 g/L Leu/Val, 0.5 g/L K₂HPO₄, 0.1 g/L MgSO₄, 50 mg/L Fe₂(SO₄)₃, 100 mM MOPS

Further culture optimization, production of PF analogues

This work

LA

Modified Landy’s

20 g/L glucose, 2.3 g/L (NH₄)₂SO₄, 2 g/L Glu, 0.5 g/L MgSO₄, 1.6 mg/L CuSO₄, 1.2 mg/L Fe₂(SO₄)₃, 0.4 mg/L MnSO₄, 1 g/L yeast extract, 100 mM MOPS

Initial media screening

Modified from [39]

LB

Lysogeny broth

10 g/L tryptone, 5 g/L yeast extract, 10 g/L NaCl

Seed cultures, initial media screening

–

MSM

Mineral salt medium

20 g/L glucose, 2.3 g/L (NH₄)₂SO₄, 0.5 g/L sodium citrate, 2 g/L K₂HPO₄, 0.1 g/L MgSO₄, 100 mM MOPS

Initial media screening

Modified from [17]