Table 5 CCD experimental design in the Biolector®
Variable levels | Variable (g/L) | Max. DCW (g/L) | Max. PFC (mg/L) | Max. YP/X (mg/g) | ||
|---|---|---|---|---|---|---|
A | B | C | ||||
Glycerol | Tryptone | Leu | ||||
Low star point (− α): | 16 | 3.9 | 0.8 | |||
Low (−): | 50 | 8.0 | 2.5 | |||
Medium (0): | 100 | 14.0 | 5.0 | |||
High (+): | 150 | 20.0 | 7.5 | |||
High star point (+ α): | 184 | 24.1 | 9.2 | |||
Experimental run | ||||||
1 | 0 | − α | 0 | 9.43 ± 0.14 | 224.3 ± 13.3 | 23.8 ± 1.3 |
2 | 0 | 0 | 0 | 15.41 ± 0.22 | 397.9 ± 16.4 | 25.8 ± 1.3 |
3 | 0 | 0 | 0 | 15.74 ± 0.25 | 411.6 ± 14.6 | 26.1 ± 1.0 |
4 | 0 | 0 | 0 | 16.05 ± 0.30 | 425.1 ± 7.5 | 26.5 ± 0.6 |
5 | − α | 0 | 0 | 29.64 ± 0.80 | 200.2 ± 10.1 | 9.5 ± 0.5 |
6 | − | − | − | 22.28 ± 0.37 | 137.9 ± 14.2 | 6.2 ± 0.7 |
7 | 0 | 0 | − α | 16.70 ± 0.14 | 236.6 ± 12.7 | 14.2 ± 0.8 |
8 | 0 | 0 | 0 | 16.78 ± 0.19 | 396.2 ± 9.5 | 23.6 ± 0.6 |
9 | 0 | + α | 0 | 20.01 ± 0.38 | 382.5 ± 10.7 | 19.1 ± 0.4 |
10 | − | + | − | 30.93 ± 0.79 | 189.6 ± 12.7 | 6.1 ± 0.4 |
11 | + | + | + | 0.42 ± 0.03 | 34.9 ± 6.5 | 82.7 ± 19.4 |
12 | − | + | + | 27.94 ± 0.41 | 264.0 ± 6.3 | 9.4 ± 0.2 |
13 | 0 | 0 | 0 | 15.33 ± 0.31 | 412.2 ± 9.6 | 26.9 ± 0.8 |
14 | 0 | 0 | 0 | 16.23 ± 0.33 | 392.0 ± 8.4 | 24.2 ± 0.6 |
15 | + α | 0 | 0 | 0.01 ± 0.00 | 4.4 ± 1.0 | 723.6 ± 82.8 |
16 | 0 | 0 | 0 | 16.34 ± 0.28 | 405.6 ± 11.8 | 24.8 ± 0.8 |
17 | + | − | − | 0.74 ± 0.05 | 19.1 ± 3.2 | 25.9 ± 5.0 |
18 | 0 | 0 | + α | 15.27 ± 0.29 | 640.2 ± 5.2 | 41.9 ± 0.8 |
19 | 0 | 0 | 0 | 16.68 ± 0.45 | 405.8 ± 6.7 | 24.3 ± 0.9 |
20 | 0 | 0 | 0 | 15.95 ± 1.09 | 409.9 ± 12.3 | 25.7 ± 2.1 |
21 | + | − | + | 0.14 ± 0.02 | 9.5 ± 2.4 | 68.0 ± 24.1 |
22 | + | + | − | 0.18 ± 0.02 | 21.5 ± 3.5 | 122.3 ± 22.3 |
23 | − | − | + | 25.44 ± 0.32 | 438.2 ± 13.0 | 17.2 ± 0.6 |