High-throughput optimization of medium components and culture conditions for the efficient production of a lipopeptide pseudofactin by Pseudomonas fluorescens BD5

Table 3 Maximal growth (DCW), PF concentration (PF_C), and specific PF productivity (Y_P/X) in cultures during testing of various complex nitrogen additives using the Biolector^® after culture for 66 h

Medium	Max. DCW (g/L)	Max. PF_C (mg/L)	Max. Y_P/X (mg/g)
KB-M2 +peptone	9.13 ± 0.31	514.0 ± 3.8	57.49 ± 1.27
KB-M2 +proteose peptone	6.01 ± 0.30	327.9 ± 11.7	54.98 ± 3.09
KB-M2 +yeast extract	21.86 ± 0.71	515.4 ± 10.7	23.86 ± 0.32
KB-M2 +tryptone	11.16 ± 0.33	628.7 ± 9.5	56.30 ± 1.89
KB-M2 +soy peptone	19.37 ± 0.48	77.6 ± 7.7	4.03 ± 0.23
KB-M2 +casitone	8.96 ± 0.44	397.2 ± 5.6	44.91 ± 1.68

All results are shown as the mean of three replicates ± SD. Culture media are summarized in Table 1 and in “Culture media” section

ISSN: 1475-2859