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Table 2 Maximal growth (DCW), PF concentration (PFC), and specific PF productivity (YP/X) in the cultures during first medium screening in the Biolector® after culture for 48 h

From: High-throughput optimization of medium components and culture conditions for the efficient production of a lipopeptide pseudofactin by Pseudomonas fluorescens BD5

Medium

OTR = 30 mmol/L/h

OTR = 15 mmol/L/h

Max. DCW (g/L)

Max. PFC (mg/L)

Max. YP/X (mg/g)

Max. DCW (g/L)

Max. PFC (mg/L)

Max. YP/X (mg/g)

KB

5.66 ± 0.12

32.8 ± 1.3

7.77 ± 0.55

5.11 ± 0.35

28.6 ± 0.9

6.82 ± 0.14

KB-M1

5.17 ± 0.30

22.1 ± 0.9

5.03 ± 0.37

4.50 ± 0.15

18.7 ± 1.2

4.40 ± 0.37

KB-M1 +Leu

4.72 ± 0.43

57.9 ± 0.8

12.59 ± 0.41

4.51 ± 0.20

54.1 ± 0.5

12.38 ± 0.64

KB-M1 +Glu

5.25 ± 0.49

21.7 ± 1.3

6.46 ± 0.10

4.63 ± 0.26

20.0 ± 1.0

5.93 ± 0.24

KB-M1 +AA mix

3.97 ± 0.30

30.2 ± 0.8

8.18 ± 0.14

3.73 ± 0.37

28.8 ± 0.5

8.08 ± 0.27

KB-M1 +Am

4.25 ± 0.26

8.5 ± 0.9

2.02 ± 0.16

3.69 ± 0.49

7.6 ± 1.3

2.07 ± 0.35

KB-M1 +Leu+Am

4.76 ± 0.20

12.1 ± 1.0

2.68 ± 0.24

4.71 ± 0.07

10.2 ± 0.3

2.38 ± 0.14

KB-M1 +Glu+Am

4.95 ± 0.12

8.9 ± 1.3

2.02 ± 0.28

4.97 ± 0.23

8.1 ± 0.5

1.88 ± 0.05

KB-M1 +Cit

2.38 ± 0.39

2.4 ± 0.4

1.05 ± 0.22

2.47 ± 0.41

1.8 ± 1.0

0.72 ± 0.37

KB-M1 +Suc

3.32 ± 0.30

7.7 ± 0.2

2.72 ± 0.10

3.59 ± 0.18

3.2 ± 1.5

1.18 ± 0.52

  1. All results are shown as the mean of three replicates ± SD. Culture media are summarized in Table 1 and in “Culture media” section
  2. OTR oxygen transfer rate