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Table 1 Maximal growth (DCW), PF concentration (PFC), specific PF productivity (YP/X), and pH during initial medium screening after 168 h of culture

From: High-throughput optimization of medium components and culture conditions for the efficient production of a lipopeptide pseudofactin by Pseudomonas fluorescens BD5

Medium

Max. DCW (g/L)

Max. PFC (mg/L)

Max. YP/X (mg/g)

Final pH

MSM

2.80 ± 0.13

10.1 ± 0.5

4.81 ± 0.28

4.1 ± 0.1

MSM +T

2.65 ± 0.24

11.8 ± 0.5

4.88 ± 0.58

4.0 ± 0.2

MSM +LB

3.89 ± 0.10

16.4 ± 0.3

5.04 ± 1.13

4.2 ± 0.2

MSM +G

2.46 ± 0.22

15.4 ± 1.0

6.51 ± 1.00

5.2 ± 0.1

MSM +G+LB

3.76 ± 0.06

20.9 ± 0.5

6.94 ± 0.65

5.3 ± 0.1

MSM +H

3.01 ± 0.38

4.2 ± 1.3

1.40 ± 0.46

6.6 ± 0.2

MSM +O

3.17 ± 0.63

4.7 ± 0.7

1.54 ± 0.24

6.7 ± 0.2

LB

10.10 ± 0.07

20.4 ± 0.6

2.37 ± 0.31

6.5 ± 0.1

KB

12.57 ± 0.09

80.8 ± 0.8

7.75 ± 0.25

7.1 ± 0.1

LA

7.31 ± 0.21

6.9 ± 0.8

1.48 ± 0.25

4.2 ± 0.1

LA +G

9.47 ± 0.18

23.0 ± 0.6

2.47 ± 0.08

6.5 ± 0.2

  1. All results are shown as the mean value of three replicates ± SD. Culturing media are summarized in Table 6 and in “Culture media” section