Fig. 3From: Spatial organization of enzymes to enhance synthetic pathways in microbial chassis: a systematic reviewTypical scaffolding approaches based on genome editing tools. a ZFP-based method using synthesized plasmid DNA containing binding motifs of ZFPs as medium molecule, enzymes are fused with zinc finger domains. b TALE based approach using DNA containing multiple binding motifs of TALEs as medium molecule. Enzymes are fused with artificial designed and constructed TALE domains with high DNA-binding affinity and specificity. c CRISPR-Cas9 system and its potential on spatial organization of enzymesBack to article page