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Fig. 4 | Microbial Cell Factories

Fig. 4

From: Metabolic engineering of Corynebacterium glutamicum for the production of cis, cis-muconic acid from lignin

Fig. 4

Production of ciscis-muconic acid (MA) with feeding of catechol. The production was conducted in a shake flask. Metabolically engineered Corynebacterium glutamicum MA-2 was grown on glucose minimal medium for the first 2 h. Then, the feeding was started. Every hour, pulses with 5 mM catechol and 0.5 g L−1 glucose, were added. In addition, the pH was controlled above 7.0 by manual control, adding appropriate volumes of 10 mM NaOH. The black arrow indicates the time point, when the glucose feed was stopped. The data represent mean values and standard deviations from three biological replicates

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