Presence of protein production enhancers results in significantly higher methanol-induced protein production in Pichia pastoris

Table 1 Summary of Pichia pastoris alcohol oxidase promoter regulatory elements

Regulatory elements	Description	Classification	Refs
MxrI	Transcription factor involved in the activation of the methanol metabolism genes	Cis	[31]
Region-D	Transcription factor binding site involved in the binding of the transcription factor AdrIp	Cis	[6]
Putative TFBS regions	AOX1 promoter library generated by systematic deletion of sequences within the PAOX1	Cis	[5]
Protein 14.3.3	Binds to Mxr1P and further inhibits all Mxr1p regulated gene repression	Trans	[32]
Prm1	Constitutive expression of the PRM1 results in activation of the PAOX1 in methanol free medium	Trans	[33]
Mit1	MIT1 regulates methanol induction of PAOX1	Trans	[34]
HXT1	Pp HXT1 is necessary for catabolite repression of PAOX1	Trans	[35]
ZTA1	Single strand DNA binding protein, which regulates PAOX1 during oxidative stress	Trans	[36]
5′ UTR	4-nucleotide sequence immediately preceding AUG start codon A-rich	Kozak sequence	[10]
PAOX1-5′ UTR	Several regions identified affecting translation positively as well as negatively	Cis	[14]

Cis regulatory elements are regions within the PAOX1 that act as a transcriptional factor binding sites, Trans regulatory elements are regulatory proteins/transcriptional factors that bind to the cis regulatory regions

ISSN: 1475-2859