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Fig. 1 | Microbial Cell Factories

Fig. 1

From: Presence of protein production enhancers results in significantly higher methanol-induced protein production in Pichia pastoris

Fig. 1

Protein yield represented relative to median value of reference strains. Effect of putative transcription and translation enhancing elements on the production yield of feruloyl esterase (FAE) by Pichia pastoris GS115. Microtiter scale protein production yield of ten transformants selected by histidine complementation after 72 h of methanol induction in BMMy. FAE protein yield was quantified by the specific rate of the enzyme solution to hydrolyse 4-nitrophenyl ferulate. Dots represent production data from individual transformants. To compare element and element combinations performance median yield values are used which are indicated by a horizontal line. UNA1 and UNA2 represent the regulatory elements upstream of the PAOX1, UNB represents a modified 5′UTR. UNall represent the combination of regulatory elements UNA1, UNA2, and UNB

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Microbial Cell Factories

ISSN: 1475-2859

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