Fig. 2
The cry5Ba3Φ gene confers strong nematicidal activity to Botrytis cinerea. a Diet fungus of pinewood nematode (PWN) in the laboratory. Decreased number of live nematodes (a 24 h, one-way ANOVA, F4,20 = 28.227, P < 0.0001; b 48 h, one-way ANOVA, F4,20 = 12.319, P < 0.0001) and live:dead ratio (c 24 h, one-way ANOVA, F4,20 = 11.735, P < 0.0001; d 48 h, one-way ANOVA, F4,20 = 11.168, P < 0.0001) with elevated proportion of extracts from cry5Ba3Φ-transgenic Botrytis cinerea. Fitness loss of PWN population (e number of live nematodes, t = 5.039, df = 10, P < 0.001; f dead:live ratio, t = − 9.573, df = 5.008, P < 0.001) feeding on mycelia of cry5Ba3Φ-transgenic Botrytis cinerea, after 5 days. *** Indicates P < 0.001. Different letters indicate significant differences among treatments (P < 0.05)