Fig. 1

Recombination of cry5Ba3Φ-transgenic Botrytis cinerea. a Construction of the plasmid pTFCM-cry5Ba3Φ. HYG: hygromycin B resistant (hygromycin B phosphotransferase) gene; PtrpC and TtrpC: promoter and terminator of Aspergillus nidulans, respectively. b Plasmid pUC57-cry5Ba3Φ digested by SpeI and XhoI. M: DNA marker; 1: plasmid pUC57; 2: plasmid pUC57-cry5Ba3Φ. c Certification of the plasmid pTFCM-cry5Ba3Φ. M: DNA marker; 1–2: plasmid pTFCM-cry5Ba3Φ digested by SacI and XhoI; 3–4: plasmid pTFCM-cry5Ba3Φ digested by SpeI and XhoI. d Identification of the AGL-1 pTFCM-cry5Ba3Φ by PCR amplification with cry-F/cry-R primers. M: DNA Marker; 1: plasmid pTFCM-cry5Ba3Φ; 2–4: AGL-1 pTFCM-cry5Ba3Φ; 5: AGL-1 pTFCM. e Southern blot analysis of genomic DNA of cry5Ba3Φ-transgenic Botrytis cinerea. DNA was digested with HindIII and probed with cry5Ba3Φ. 1: cry5Ba3Φ-transgenic Botrytis cinerea; 2: wild-type Botrytis cinerea; 3: plasmid pTFCM-cry5Ba3Φ. f Confirmation of cry5Ba3Φ-transgenic Botrytis cinerea by PCR amplification with cry-F/cry-R primers. M: DNA marker; 1: cry5Ba3Φ-transgenic Botrytis cinerea; 2: plasmid pTFCM-cry5Ba3Φ; 3: wild-type Botrytis cinerea. g Expression quantity of cry5Ba3Φ gene in cry5Ba3Φ-transgenic and wild-type Botrytis cinerea strains (P < 0.05). h SDS-PAGE analysis of soluble proteins produced by cry5Ba3Φ-transgenic Botrytis cinerea. An asterisk indicates the protein band of Cry5Ba3Φ protein. MW: protein molecular weight; M: pre-stained protein marker; C: Botrytis cinerea (pTFCM); T: Botrytis cinerea (pTFCM-cry5Ba3Φ)