Table 4 Fermentation products of E. coli mutants in mineral medium with glucose as the carbon source
From: Engineering of Escherichia coli for Krebs cycle-dependent production of malic acid
Strain | Additional genotype modifications | Plasmid | Cells (g/g) | Malate (mol/mol) | Fumarate (mol/mol) | Pyruvate (mol/mol) | Acetate (mol/mol) |
|---|---|---|---|---|---|---|---|
Sy502 | – | pACT3w-ppcK620S | 0.20 ± 0.06 | 0.40 ± 0.08 | 0.09 ± 0.03 | 0.00 | 0.56 ± 0.03 |
Sy504 | ΔiclR | pACT3w-ppcK620S | 0.19 ± 0.05 | 0.42 ± 0.05 | 0.10 ± 0.04 | 0.00 | 0.55 ± 0.06 |
Sy510 | ΔiclR ΔackA | pACT3w-ppcK620S | 0.21 ± 0.01 | 0.55 ± 0.02 | 0.11 ± 0.01 | 0.01 ± 0.00 | 0.43 ± 0.00 |
Sy508 | ΔiclR ΔpoxB | pACT3w-ppcK620S | 0.16 ± 0.02 | 0.30 ± 0.07 | 0.07 ± 0.03 | 0.11 ± 0.02 | 0.29 ± 0.22 |
Sy512 | ΔiclR ΔackA ΔpoxB | pACT3w-ppcK620S | 0.22 ± 0.03 | 0.35 ± 0.00 | 0.10 ± 0.01 | 0.56 ± 0.01 | 0.02 ± 0.00 |
Sy729 | ΔiclR ΔarcA | pACT3w-ppcK620S | 0.15 ± 0.02 | 0.53 ± 0.03 | 0.06 ± 0.04 | 0.04 ± 0.03 | 0.38 ± 0.04 |
Sy731 | ΔiclR ΔarcA | pACT3w-ppcK620S-gltAR164L | 0.15 ± 0.02 | 0.82 ± 0.07 | 0.06 ± 0.02 | 0.02 ± 0.04 | 0.25 ± 0.09 |