Table 3 Fermentation products of E. coli MG1655 mutants in mineral medium with glucose as the carbon source
From: Engineering of Escherichia coli for Krebs cycle-dependent production of malic acid
Strain | Genotype | Plasmid | Cells (g/g) | Malate (mol/mol) | Fumarate (mol/mol) | Pyruvate (mol/mol) | Acetate (mol/mol) |
|---|---|---|---|---|---|---|---|
K12 | Wild-type | – | 0.32 ± 0.02 | 0.01 ± 0.01 | 0.00 | 0.28 ± 0.10 | 0.48 ± 0.04 |
Sy62 | Δmdh | – | 0.27 ± 0.02 | 0.06 ± 0.00 | 0.00 | 0.17 ± 0.02 | 0.88 ± 0.15 |
Sy111 | Δmqo | – | 0.33 ± 0.03 | 0.06 ± 0.03 | 0.00 | 0.18 ± 0.01 | 0.34 ± 0.08 |
Sy68 | Δmdh Δmqo | – | 0.27 ± 0.02 | 0.04 ± 0.00 | 0.00 | 0.15 ± 0.02 | 1.31 ± 0.20 |
Sy102 | Δmdh Δmqo ΔackA-pta | – | 0.23 ± 0.02 | 0.01 ± 0.00 | 0.00 | 1.30 ± 0.05 | 0.21 ± 0.02 |
Sy162 | Δmdh Δmqo ΔackA-pta ΔmaeA | – | 0.22 ± 0.02 | 0.03 ± 0.02 | 0.00 | 0.17 ± 0.02 | 0.79 ± 0.02 |
Sy168 | Δmdh Δmqo ΔackA-pta ΔmaeA ΔmaeB | – | 0.16 ± 0.06 | 0.12 ± 0.04 | 0.06 ± 0.02 | 0.24 ± 0.04 | 1.06 ± 0.25 |
Sy279 | Δmdh Δmqo ΔackA-pta ΔmaeA ΔmaeB | pACT3w-ppcwt | 0.12 ± 0.03 | 0.13 ± 0.02 | 0.04 ± 0.01 | 0.95 ± 0.02 | 0.69 ± 0.10 |
Sy320 | Δmdh Δmqo ΔackA-pta ΔmaeA ΔmaeB | pEXT20s-ppcK620S | 0.18 ± 0.01 | 0.23 ± 0.01 | 0.03 ± 0.01 | 0.06 ± 0.03 | 1.65 ± 0.09 |
Sy321 | Δmdh Δmqo ΔackA-pta ΔmaeA ΔmaeB | pEXT20w-ppcK620S | 0.13 ± 0.01 | 0.14 ± 0.03 | 0.05 ± 0.04 | 0.96 ± 0.03 | 0.77 ± 0.08 |
Sy322 | Δmdh Δmqo ΔackA-pta ΔmaeA ΔmaeB | pACT3s-ppcK620S | 0.28 ± 0.02 | 0.37 ± 0.02 | 0.14 ± 0.01 | 0.00 ± 0.00 | 0.64 ± 0.01 |
Sy280 | Δmdh Δmqo ΔackA-pta ΔmaeA ΔmaeB | pACT3w-ppcK620S | 0.21 ± 0.00 | 0.48 ± 0.02 | 0.11 ± 0.02 | 0.28 ± 0.05 | 0.33 ± 0.04 |