Rational modification of tricarboxylic acid cycle for improving l-lysine production in Corynebacterium glutamicum

Table 4 The DCW, l-lysine production, maximal specific growth rate (μ_max.), and maximal specific production rate of l-lysine (q_{Lys, max.}) of genetically defined C. glutamicum strains in the shaking culture and under the different concentration of biotin

Biotin conc. (mg L⁻¹)	JL-69P_tac-M gdh				JL-69P_tuf gdh				JL-69P_sod gdh
Biotin conc. (mg L⁻¹)	Lys conc. (g L⁻¹)	DCW (g L⁻¹)	μ_max (h⁻¹)	q_{Lys, max.}(g g⁻¹ h⁻¹)	Lys conc. (g L⁻¹)	DCW (g L⁻¹)	μ_max (h⁻¹)	q_{Lys, max} (g g⁻¹ h⁻¹)	Lys conc. (g L⁻¹)	DCW (g L⁻¹)	μ_max (h⁻¹)	q_{Lys, max.} (g g⁻¹ h⁻¹)
0.2	23.86 ± 2.16	7.53 ± 1.12	0.17	0.63 ± 0.11	16.03 ± 1.32	5.36 ± 1.04	0.14	0.81 ± 0.17	20.16 ± 2.08	6.78 ± 0.72	0.16	0.70 ± 0.08
0.3	23.93 ± 1.28	8.72 ± 1.04	0.19	0.61 ± 0.06	17.10 ± 1.23	7.71 ± 1.36	0.18	0.64 ± 0.04	20.63 ± 3.15	8.03 ± 1.02	0.19	0.67 ± 0.13
0.4	24.36 ± 2.11	9.25 ± 1.13	0.23	0.63 ± 0.14	19.24 ± 1.48	8.82 ± 1.44	0.21	0.65 ± 0.12	21.32 ± 1.77	9.10 ± 1.21	0.22	0.67 ± 0.05
0.5	25.05 ± 2.16	9.76 ± 1.09	0.23	0.69 ± 0.13	20.21 ± 1.12	9.38 ± 0.95	0.22	0.63 ± 0.20	22.44 ± 2.13	10.07 ± 0.58	0.26	0.65 ± 0.14
0.6	25.24 ± 1.74	10.23 ± 2.04	0.25	0.68 ± 0.11	21.26 ± 2.05	10.16 ± 1.01	0.25	0.66 ± 0.13	24.05 ± 1.66	10.38 ± 0.42	0.26	0.66 ± 0.03
0.8	24.47 ± 2.18	11.35 ± 1.18	0.28	0.62 ± 0.16	22.47 ± 1.32	10.83 ± 2.11	0.29	0.67 ± 0.07	24.21 ± 1.05	11.11 ± 1.03	0.31	0.63 ± 0.09
1.0	21.83 ± 2.10	13.14 ± 1.05	0.33	0.56 ± 0.05	20.82 ± 1.74	12.96 ± 1.02	0.33	0.59 ± 0.04	22.15 ± 2.12	12.76 ± 1.15	0.32	0.62 ± 0.11
1.2	18.29 ± 1.93	16.59 ± 2.06	0.39	0.43 ± 0.08	19.13 ± 1.02	15.38 ± 1.17	0.34	0.50 ± 0.12	21.02 ± 1.76	15.49 ± 1.26	0.35	0.55 ± 0.06

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